All displayed gRNA spacer classes are highly specific in regard to other NGG sites. Class A0, B0, A0.1 and B0.1 gRNA spacer sequences are also highly specific in regard to NAG sites. Class A1, B1, A2 and B2 have an off-target potential to NAG, but not NGG, sites.

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Two RGE vectors developed by Yang's Lab are available via Addgene: Yang's Lab Addgene site. Our polycistronic-tRNA-gRNA technology provides very efficient multiplex genome editing. The supplemental information contains a detailed protocol on design and use.

Working with a different genome or Cas variant? You can clone the pipeline used for analysis from our GitHub repository and customize it based on your needs!