Welcome to CRISPR-PLANT!
CRISPR-PLANT helps researchers using the CRISPR-Cas9 system for plant genome editing. This website you are currently on is an alternative version. The AGI hosts the original website as it was when the article was published at http://omap.org/crispr2/.
Working with a different genome or Cas variant? You can clone the pipeline used for analysis from our GitHub repository and customize it based on your needs.
What is CRISPR-Cas9?
CRISPR-Cas9 is a type II CRISPR-Cas system. The CRISPR-Cas9 system from Streptococcus pyogenes is a simple and versatile tool for RNA-guided genome editing (RGE) in different organisms.
During Cas9-mediated RGE, a short guide RNA (gRNA) directs Cas9 to target a DNA site for editing or transcriptional control. The gRNA-Cas9 complex recognizes the DNA through base pairing between the gRNA spacer and the complementary DNA strand. A PAM (protospacer adjacent motif) must also be present adjacent to the target. While highly programmable, imperfect matches can lead to off-target effects. This website provides a resource of highly specific gRNA spacer sequences for seven different plant species.
What is CRISPR-PLANT?
CRISPR-PLANT is a platform to help researchers design and construct specific gRNAs for CRISPR-Cas9-mediated genome editing in plants. It offers pre-analyzed gRNA spacers with specificity data for seven model and crop species.
Start searching CRISPR gRNA spacers to start using CRISPR-PLANT v2.
Learn more about how CRISPR-Cas9 works in plants if you're new to CRISPR-Cas9.
News & Updates
- 05/20/2025 – Updated style of website and improved Search (incl. the ability to download results as CSV file)
- 09/27/2018 – Added information to CRISPR-PLANT GitHub repo
- 11/01/2017 – Update to CRISPR-PLANT v2 by BM and JWZ
- 10/21/2013 – CRISPR-PLANT 0.1 was online
- 10/15/2013 – Demo version was set up